Lea Blood Group Antigen on Human Platelets

Open Access

1 January 1985

journal article
research article
Published by Oxford University Press (OUP) in American Journal of Clinical Pathology

Vol. 83 (1) , 90-94
https://doi.org/10.1093/ajcp/83.1.90

Abstract

One- and two-stage radioligand assays were used to determine if human platelets possess the Le^a antigen. Goat IgG anti-Le^a antibody was purified by multiple adsorptions with Le(a−b−) human red blood cells, followed by affinity chromatography with synthetic Le^a substance and labeling with ¹²⁵I. Human IgG anti-Le^a antibody was used either in a two stage radioassay with ¹²⁵I-labeled mouse monoclonal IgG anti-human IgG as the second antibody or, alternatively, purified by Staph protein A chromatography, labeled with ¹²⁵I, and used in a one-stage radioassay. Platelets from donors of appropriate red blood cell phenotypes were incubated with the antisera, centrifuged through phthalate esters, and assayed in a gamma scintillation counter. Dose response and saturation curve analysis demonstrate the presence of Lewis a antigen on platelets from Le^a+ donors. Furthermore, platelets from an Le(a − b−) donor incubated in Le (a+b−) plasma adsorb Le^a antigen in a similar manner to red blood cells. The clinical significance of these antigens in platelet transfusion remains undefined.

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