Thrombospondin 1 binds to the surface of bovine articular chondrocytes by a linear RGD‐dependent mechanism
- 24 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 363 (3) , 214-216
- https://doi.org/10.1016/0014-5793(95)00311-v
Abstract
Thrombospondin 1 is present in articular cartilage and is synthesized by chondrocytes. Adult bovine articular chondrocytes in serum-free medium were evaluated in a solid-phase assay for their ability to attach to thrombospondin 1 isolated from human platelets. The chondrocytes attached to the thrombospondin 1 by a mechanism that was inhibited by a synthetic linear GRGDSP but not a GRGESP peptide. The cells, however, did not spread on thrombospondin 1, but did spread on fibronectin and Pep-Tite 2000, a synthetic RGD-containing peptide. Preincubation of thrombospondin 1 with EDTA irreversibly inhibited its capacity to attach to chondrocytes. We conclude that thrombospondin 1 binds to chondrocytes by its RGD sequenceKeywords
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