Evidence for new phosphorylation sites for protein kinase C and cyclic AMP‐dependent protein kinase in bovine heart 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase
- 28 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 310 (2) , 139-142
- https://doi.org/10.1016/0014-5793(92)81315-d
Abstract
Bovine heart 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase (PFK‐2/FBPase‐2) was phosphorylated by incubation with [γ‐32P]MgATP and cyclic AMP‐dependent protein kinase (PKA) or protein kinase C (PKC). After digestion with chymotrypsin, the phosphorylation sites for the two protein kinases were identified by peptide mapping, and microsequencing. Evidence for new phosphorylation sites for PKA (Ser‐483) and PKC (Ser‐84 and Ser‐466) was obtained.Keywords
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