Nearly identical members of the heterogeneous LAP gene family are expressed in thymus of different mouse strains
Open Access
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (9) , 3823-3834
- https://doi.org/10.1093/nar/15.9.3823
Abstract
Poly (A)RNAs prepared from the thymuses of C57BL/6J and DBA/2J mice were used to construct cDNA libraries in the bacterial expression vector Agt11. The libraries were scanned first for protein production with polyvalent antiserum prepared against the 73kDa gag protein of mouse intracisternal A-particles (IAP). Reactive plaques were crossed-screened by hybridization with an IAP-specific DNA probe. Two IAP-specific protein-producing plaques were obtained from the C57BL/6 library and 4 from the DBA/2 library. One C57BL/6 cDNA clone (B12) and two DBA cDNA clones (D8 and D20) were sequenced in their entirety. Clones B12 and D8 were remarkably similar, particularly when compared to the 6 other IAP elements that have been sequenced thus far. We discuss the evidence which leads us to suggest that these clones may be derived from allelic IAP elements expressed in mouse thymus.Keywords
This publication has 24 references indexed in Scilit:
- A nonpolymorphic class I gene in the murine major histocompatibility complexCell, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Efficient isolation of genes by using antibody probes.Proceedings of the National Academy of Sciences, 1983
- A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.Molecular and Cellular Biology, 1983
- Formation of rat IgE-binding factors by rat-mouse T cell hybridomas.The Journal of Immunology, 1982
- Sequence organization of cloned intracisternal a particle genesCell, 1980
- Intracisternal A-particle genes: identification in the genome of Mus musculus and comparison of multiple isolates from a mouse gene library.Proceedings of the National Academy of Sciences, 1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Sequences associated with intracisternal a particles are reiterated in the mouse genomeCell, 1977
- Sequence of amino acids in the major and minor β chains of the diffuse hemoglobin from BALB/c miceBiochimica et Biophysica Acta (BBA) - Protein Structure, 1973