Pigment-Protein Complexes of Illuminated Etiolated Leaves
- 1 October 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 70 (4) , 1019-1025
- https://doi.org/10.1104/pp.70.4.1019
Abstract
Photoconversion of protochlorophyllide in etiolated leaves of Avena sativa L., cultivars Pennal and Peniarth and Phaseolus vulgare L., cv. The Prince results in the sequential appearance of spectrally distinct chlorophyllide complexes (Chlide 678, 684 and 672). The generation of similar forms in vitro, under controlled conditions, using well characterized etioplast membranes enriched in the enzyme protochlorophyllide [Pchlide] reductase is reported. Excess NADP+ and NADPH stabilize complexes related to Chlide 678 and Chlide 684, respectively, whereas addition of exogenous Pchlide induces formation of a species related to Chlide 672. Apparently, Chlide 678 and Chlide 684 represent ternary complexes of the enzyme protochlorophyllide reductase, with Chlide and either NADP+ (Chlide 678) or NADPH (Chlide 684). Chlide 672 is seen as free pigment dissociated from the enzyme. The role of Pchlide in this dissociation, observed spectroscopically as the Shibata shift, is discussed.This publication has 16 references indexed in Scilit:
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