FUNCTIONAL-PROPERTIES OF CONTINUOUSLY CULTURED HUMAN LYMPHOCYTES-T

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (2) , 329-335
Abstract
Human T lymphocytes (CCTC) were maintained in continuous culture by a new method. CCTC ability to produce 3 types of T cell response was determined and compared to the published responses of T cells grown in T cell growth factor (TCGF) medium. Unlike TCGF cells, CCTC will not give proliferative responses when stimulated with phytohemagglutinin, concanavalin A, or allogeneic lymphocytes even when supplemented with autologous peripheral blood lymphocytes as a source of accessory cell. Instead, CCTC are potent inhibitors of the normal cell proliferative response in MLC. This suppressive activity is not specific for histocompatible cells. CCTC express cytotoxic activity to a number of human lymphoid cell lines and this activity appears to be different from the polyclonal cytotoxicity reported for TCGF-maintained cells. Long-term T cell culture appears to grow cells with different properties from TCGF cells.