Structure and Thickness Dependence of “Molecular Wiring” in Nanostructured Enzyme Multilayers
- 8 December 2005
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (2) , 399-407
- https://doi.org/10.1021/ac050973n
Abstract
Supramolecular organized multilayers composed of glucose oxidase (GOx) and osmium-derivatized poly(allylamine) redox polymer have been self-assembled electrostatically from Os-polyelectrolyte solutions of variable pH (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-by-layer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of beta-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed.Keywords
This publication has 34 references indexed in Scilit:
- Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation proceduresReviews in Molecular Biotechnology, 2002
- Layer-By-Layer Self-Assembly of Glucose Oxidase and Os(Bpy)2ClPyCH2NH−poly(Allylamine) BioelectrodeAnalytical Chemistry, 2001
- Step-by-Step Avidin−Biotin Construction of Bienzyme Electrodes. Kinetic Analysis of the Coupling between the Catalytic Activities of Immobilized Monomolecular Layers of Glucose Oxidase and HexokinaseLangmuir, 1999
- Use of Mixed Self-Assembled Monolayers in a Study of the Effect of the Microenvironment on Immobilized Glucose OxidaseLangmuir, 1999
- Electrical contact of redox enzyme layers associated with electrodes: Routes to amperometric biosensorsElectroanalysis, 1997
- Layer-by-Layer Self-Assembly of Glucose Oxidase with a Poly(allylamine)ferrocene Redox MediatorLangmuir, 1997
- From Homogeneous Electroenzymatic Kinetics to Antigen−Antibody Construction and Characterization of Spatially Ordered Catalytic Enzyme Assemblies on ElectrodesAccounts of Chemical Research, 1996
- Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilterJournal of Fermentation and Bioengineering, 1996
- Application of Redox Enzymes for Probing the Antigen−Antibody Association at Monolayer Interfaces: Development of Amperometric Immunosensor ElectrodesAnalytical Chemistry, 1996
- Electrical wiring of redox enzymesAccounts of Chemical Research, 1990