Determination of branched-chain L-amino acids and their keto analogs with leucine dehydrogenase.
Open Access
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 42 (10) , 1919-1922
- https://doi.org/10.1271/bbb1961.42.1919
Abstract
The simple and sensitive spectrophotometric method for determination of branchedchain L-amino acids and their keto analogs with leucine dehydrogenase (EC 1.4.1.9) is described. Branched-chain L-amino acids were determined with the enzyme by measuring the formation of NADH spectrophotometrically. A linear relationship was established between the absorbance at 340nm and the concentration of the amino acid (0.02_??_0.20 μmol). The determination of branched-chain keto acids (0.01_??_0.12 μmol) is based on the measurement of NADH disappeared. This method can be used to assay D-amino acid aminotransferase (EC 2.6.1.21), and is applicable to the assay of other enzymes catalyzing the formation or decomposition of branched-chain L-amino acids and their keto analogs as well.Keywords
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