Infrared dichroism of the DNA-caffeine complex. A new method for determination of the ligand orientation
Open Access
- 1 December 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (12) , 3331-3336
- https://doi.org/10.1093/nar/3.12.3331
Abstract
Infrared linear dichroism (LD) measurements on films of the DNA-caffeine complex in terms of the relative humidity (r.h.) show two main effects. Firstly, there is an insertion of caffeine molecules into the DNA double helix (B form), as evidenced by a very strong parallel LD behaviour of the 745 cm−1 band due to the C-H out-of-plane deformation vibration of caffeine. Furthermore, at high r.h. values a modified B form occurs in the complex similar to the B form recently reported by BRAHMS and coworkers for DNA-polypeptide complexes. The reversible B-A transition of the DNA in dependence of the r.h. is not affected in general in the presence of caffeine.Keywords
This publication has 6 references indexed in Scilit:
- Evidence for B-C transition in ultraviolet-irradiated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Structural investigations on DNA·protamine complexesBiopolymers, 1976
- Direct Evidence of the C-Like Form of Sodium DeoxyribonucleateProceedings of the National Academy of Sciences, 1973
- THE STRUCTURE OF THE DNA-ACRIDINE COMPLEXProceedings of the National Academy of Sciences, 1963
- Structural considerations in the interaction of DNA and acridinesJournal of Molecular Biology, 1961