Pheromone Studies of the Two spotted Spider Mite. 2. Behavioral Response of Males to Quiescent Deutonymphs12

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Abstract
Adult male Tetranychus urticae Koch were attracted to quiescent female deutonymphs with the onset of quiescence. The consistency of male attraction varied from 40–85% during the early part of the period and gradually increased to 80–100% prior to adult emergence. Average duration of the quiescent period was 40.4 hours at 24°C and 23.4 hours at 30°C. Occasional males were found that did not respond consistently to quiescent deutonymphs. The cause was not determined. Male attraction was greater at 30°C and 20–30% relative humidity than at 30°C and 90–95% relative humidity. Trials at 24°C and 20–30% relative humidity were intermediate. It was concluded that tests for bioassay of T. urlicae female sex pheromone could be conducted at 30°C and 20–30% relative humidity but should include sufficient replications to measure erratic male behavior.

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