Bioluminescent Assay for Determination of Picomole Amounts of AMP, ADP, and ATP in Adipose and Muscle Tissue Biopsies

1 May 1981

journal article
Published by Georg Thieme Verlag KG in International Journal of Sports Medicine

Vol. 02 (02) , 106-109
https://doi.org/10.1055/s-2008-1034592

Abstract

A method is described for quantitative determinations of AMP, ADP, and ATP in tissue samples weighing a few milligrams by utilizing constant bioluminescent light intensity after reagent mixing. The smallest measurable quantity of adenosine nucleotide amounts to 0.2 pmol (coefficient of variation = 0.05) in the bioluminescent assay.

Keywords

AMP
ADP
ATP
BIOLUMINESCENT ASSAY
FIREFLY LUCIFERASE
ADIPOSE TISSUE BIOPSY
MUSCLE BIOPSY

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