Intratracheal Instillation Method for Mouse Lungs

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Abstract
An intratracheal instillation technique was developed which permits the repeated administrations of precise volumes of particulate suspension into mouse lungs. Benzo(a)pyrene (BP) adsorbed to ferric oxide was used to test the feasibility of the method. The majority of the animals survived 4 weekly treatments. Histological and fresh tissue sections showed extensive penetration of both chemicals into the lung parenchyma; however, while BP was eliminated from the organ rapidly, the ferric oxide was retained for over a 100 days. These dust particles were observed inside macrophages collected around terminal bronchioles and lymphoid tissues of the lungs.

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