Construction and physical map of a Bacillus subtilis specialized transducing phage .RHO.11 containing B. subtilis lys+ gene.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 28 (5) , 417-428
- https://doi.org/10.2323/jgam.28.417
Abstract
The lys+ gene was detected as the smallest DNA. fragment (2.5×106 dalton) among the several genetic markers tested from EcoRI-cleaved B. subtilis chromosomal DNA preparations by agarose gel electrophoresis followed by identification with DNA mediated transformation experiments. The DNA fragment retaining the lys+ gene was cloned in a B. subtilis temperate phage ρ11 using the prophage transformation method. The molecular weight of the genome of the constructed specialized transducing phage (ρ11dlys+) was estimated to be 72-73×106. The phage was cross-reacted with an antiserum against ρ11 phage particles. The physical map of ρ11dlys+ DNA using SalI and BamHI was quite different from that of ρ11 DNA. ρ11dlys+ did not carry theρ11-coded thymidyrate synthetase gene.This publication has 1 reference indexed in Scilit: