The fractionation of calf thymus DNA on histone KAP - Sepharose 4B columns

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Abstract
It was found that fractionation of calf thymus DNA on homologous hist-tone KAP covalently bound to CNBr activated Sepharose 4B depends on the molecular weight of DNA. The maximum of elution of high molecular DNA (m. wt. above 5 × 106) was observed at 0.56 M NaCl and that of degraded DNA (m. wt. 0.8 × 106) at 0.52 M NaCl. Significant differences in melting temperatures and melting intervals were observed among fractions obtained from low molecular DNA as a result of enrichment of some fractions in satellite DNAs. These differences were very small in DNA of m.wt. above 5 × 106. The results are discussed in terms of specific areas which may exist on calf thymus DNA molecules playing the role of loci, where lysine-rich histone KAP is preferentially bound.