Automated rate nephelometric determination of apolipoproteins AI and B in human serum by consecutive addition of antibodies

1 August 1992

journal article
Published by American Chemical Society (ACS) in Analytical Chemistry

Vol. 64 (15) , 1698-1701
https://doi.org/10.1021/ac00039a013

Abstract

A precise, rapid, automated, rate nephelometric immunoassay for apolipoprotein AI (APA) and apolipoprotein B (APB) is described. Both analytes are determined by a "one-pot" procedure which uses consecutive addition of the corresponding antibodies. Poly(oxyethylene) type nonionic surfactants are used to selectively inhibit the APB reaction after the maximum reaction rate has been reached and to enhance the APA immunoreactivity. The assay range for APA is 0.3-3.8 g/L and for APB 0.2-3.0 g/L. The new assay was compared to commercial rate nephelometric methodology. Both methods were shown to be mutually unbiased for APA and APB determinations in human serum.

Keywords

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