RACK1 inhibits the serum‐ and anchorage‐independent growth of v‐Src transformed cells

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Abstract
Cancer cells are capable of serum‐ and anchorage‐independent growth, and focus formation on monolayers of normal cells. Previously, we showed that RACK1 inhibits c‐Src kinase activity and NIH3T3 cell growth. Here, we show that RACK1 partially inhibits v‐Src kinase activity, and the serum‐ and anchorage‐independent growth of v‐Src transformed cells, but has no effect on focus formation. RACK1‐overexpressing v‐Src cells show disassembly of podosomes, which are actin‐rich structures that are distinctive to fully transformed cells. Together, our results demonstrate that RACK1 overexpression in v‐Src cells partially reverses the transformed phenotype of the cells. Our results identify an endogenous inhibitor of the oncogenic Src tyrosine kinase and of cell transformation.