STIMULATORY EFFECT OF PROLACTIN ON INCORPORATION OF [3H]THYMIDINE INTO THE URINARY BLADDER OF THE FLOUNDER (KAREIUS BICOLORATUS)

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Abstract
SUMMARY: Administration of prolactin for 3 or 5 days to the seawater-acclimatized flounder, Kareius bicoloratus, resulted in a significant increase in the incorporation of [3H]thymidine into the DNA fraction of the urinary bladder. When isolated bladders were incubated in isotonic Ringer solutions on both mucosal and serosal sides, net movement of water from mucosa to serosa decreased significantly after 3 or 5 daily injections of prolactin, whereas net absorption of sodium ions increased concomitantly; the time-course closely corresponded to that of thymidine incorporation. Tendency toward an increase in [14C]uridine and [3H]leucine incorporation was also observed in the urinary bladder after 5 daily injections of prolactin. However, incorporation of thymidine, uridine and leucine into the intestine was not affected at all by prolactin treatment for 5 days. All three isotopically labelled compounds were incorporated similarly into the urinary bladder and the intestine of control seawater flounders. Cell turnover in the urinary bladder is apparently as rapid as that in the intestine; specific stimulation of cell proliferation by prolactin may be an important factor in the induction of physiological changes in the bladder of the flounder.

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