Structural transitions of polyadenylic acid due to protonation: the influence of the length of single strands on the polarographic behaviour of the double-helical form

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Abstract
Transition of aingle-stranded poly(A) into ita double-helical protonated fora was followed by means of derivative pulae polarography, spectrophotometry, and other methods. It was found that propertied of protonated poly(A) depended on thelength of single strands from which the protonated double helixwas formed. In contrary to longer poly(A) transition of short single-atrended molecules (s20,w lower than about 3) caused practically no decrease in the pulse-polarographic current. It was concluded that the formation of the protonated double helix of poly(A) did not result in the in accesibility of the reduction site a (located in the vicinity of the surface of the molecule) for the electrode process, as it was in DNA-like double-helical polynucleotides. The current changes observed in the course of transition of longer poly(A) were explained as due to slower transport of long double-stranded molecules to the electrode.

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