Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples
- 1 March 1993
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 59 (3) , 772-776
- https://doi.org/10.1128/aem.59.3.772-776.1993
Abstract
An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by high-gradient magnetic separation. Ninety percent of the seeded cysts were recovered from buffer when this method was employed. An average of 82% of the seeded cysts were recovered from water samples with various turbidities. Significantly higher cyst recoveries were obtained from water samples with turbidities below 600 nephelometric turbidity units.Keywords
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