The reconstitution of histone H3 H4 tetramer from acid extracted histones in the absence of urea
- 16 August 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 145 (1) , 131-136
- https://doi.org/10.1016/0014-5793(82)81221-0
Abstract
Simple mixing of acid purified histones H3 and H4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high M r aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H3H4 tetramer of a structure identical to that derived from salt-extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H3H4 tetramer of closely similar structure.Keywords
This publication has 16 references indexed in Scilit:
- Lysosomal origin of chicken erythrocyte nuclear proteaseFEBS Letters, 1981
- An octamer of core histones in solution: central role of the H3.cntdot.H4 tetramer in the self-assemblyBiochemistry, 1979
- The Complete Amino‐Acid Sequence of Histone H2B(1) from Sperm of the Sea Urchin Parechinus angulosusEuropean Journal of Biochemistry, 1977
- Cross-complexing pattern of plant histonesBiochemistry, 1977
- Chapter 12 Fractionation of Histones on Molecular Sieve MatricesPublished by Elsevier ,1977
- Chapter 11 The Isolation and Purification of HistonesPublished by Elsevier ,1977
- Interaction of renatured histones f3 and f2a1Biochemical and Biophysical Research Communications, 1974
- Histone cross-complexing patternBiochemistry, 1974
- The Determination of the Primary Structure of Histone F3 from Chicken Erythrocytes by Automatic Edman DegradationEuropean Journal of Biochemistry, 1974
- A new procedure for the isolation and fractionation of histonesFEBS Letters, 1971