Quantitative separation of riboflavine from vitamin mixtures

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Abstract
A method for separating riboflavine from vitamin mixtures is described. The acidified aqueous solution is passed through a small column of large particle size talc on which the riboflavine is selectively and quantitatively adsorbed. Successive washings with 0–01 n hydrochloric acid and 5–10% dioxan remove the other components almost completely. The vitamin, eluted with 20% dioxan, is measured spectrophotometrically.

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