Site-specific mutagenesis of the human fibroblast interferon gene.
- 1 September 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (18) , 5662-5666
- https://doi.org/10.1073/pnas.81.18.5662
Abstract
Human fibroblast interferon has 3 Cys residues, located at amino acid positions 17, 31 and 141. Using the technique of site-specific mutagenesis with a synthetic oligonucleotide primer, the codon for Cys 17 was changed to a codon for Ser. The resulting interferon, IFN-.beta. Ser-17, retains the antiviral, natural killer cell activation and antiproliferative activities of native fibroblast interferon. The purified IFN-.beta. Ser-17 protein has an antiviral specific activity of 2 .times. 108 U/mg, similar to that of purified native fibroblast interferon. The purified protein is stable to long-term storage at -70.degree. C.This publication has 19 references indexed in Scilit:
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