SERUM-FREE MEDIUM FOR THE INVITRO GROWTH OF NORMAL AND MALIGNANT URINARY-BLADDER EPITHELIAL-CELLS

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (6) , 2392-2397
Abstract
A serum-free medium, DH-S1, is described which is valuable for the establishment of primary cultures of normal and malignant transitional epithelium (transitional cell carcinoma of the bladder). Growth of epithelial cells in DH-S1 is facilitated, but that of fibroblasts is suppressed. Another established human transitional cell carcinoma cell line, 647V, has grown continually in DH-S1 for over 36 passages. Morphological and antigenic studies comparing 647V cells growing in serum-containing and serum-free medium reveal differences which are pronounced at low cell density but which almost disappear at higher densities. A new human transitional cell carcinoma cell line, LA-B1, is described in which initial growth is supported by this serum-free medium.

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