Increased Interferon Production in Mice Pretreated with Corynebacterium parvum

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Abstract
C57BL/6 mice were injected intraperitoneally (i.p.) with 35 mg/kg killed C. parvum and 7-10 d later they were challenged i.p. with different interferon (IFN) inducers, including a virus (Herpes Simplex Virus, HSV), a high molecular weight polymer (poly-inosinic polycytidylic acid, poly I-C) and a low molecular weight compound (10-carboxymethyl-9-acridanone, CMA). The IFN titers in the peritoneal wash-out fluid after injection of each of the three compounds were considerably enhanced in mice pretreated by C. parvum. Furthermore, peritoneal wash-out cells (PEC) from C. parvum-treated mice were producing higher titers of IFN when stimulated in vitro with HSV or CMA than PEC from control mice. Similar data were observed when phytohemagglutinin (PHA) was used as an in vitro stimulant of IFN production. Spleen cells of C. parvum-treated mice that manifest a profound suppression of PHA-induced proliferation have no deficiency in interferon gamma IFN-γ production, but rather show a moderate enhancement. IFN induction by HSV is also slightly enhanced in spleen cell cultures of C. parvum-treated mice. Serum IFN responses to poly I-C were depressed in C. parvum-treated mice as compared to control mice, whereas the serum IFN response after i.p. injection of HSV was only slightly lower in C. parvum-treated mice than in controls.