EXCISION OF CYCLOBUTANE DIMERS IN GENOMIC AND EPISOMAL DNA IN HUMAN CELLS
- 1 September 1994
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 60 (3) , 221-224
- https://doi.org/10.1111/j.1751-1097.1994.tb05094.x
Abstract
Direct determination has been made of cyclobutyl pyrimidine dimer induction and excision repair in an episomal SV40 DNA population in vivo. Maintaining SV40-transformed human (GM637) cells in confluent culture results in amplification of a mutant SV40 episome to high copy number. T4 endonuclease V was used to quantify the induction and repair of cyclobutane dimers in the SV40 episome and genomic DNA of the same cells. Differences in both parameters were observed; cyclobutane dimers were induced at 1.5-2-fold greater frequency in episomal DNA and excised at a reduced rate compared to genomic DNA in the host cells.Keywords
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