Chiral Separations: A Comparison of Hplc and TLC
- 1 August 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (14) , 2737-2755
- https://doi.org/10.1080/01483919008049068
Abstract
Comparisons are made for separations attained in normalphase HPLC and TLC involving N-carbobenzyloxy-glycyl-L-proline and (+/−)−10-camphorsulfonic acid as chiral mobile phase additives/chiral counter ions (CMA). Possible reasons for differences in stereoselectivity of β-cyclodextrin CMA and chiral stationary phases (CSP) are discussed. In addition, differences in solubility and proposed separation mechanisms for native cyclodextrins (CD) versus derivatized CD are discussed. Inherent differences in high performance liquid chromatographic (HPLC) and thin-layer chromatographic (TLC) methods are outlined. The pros and cons of using HPLC and TLC are considered.Keywords
This publication has 20 references indexed in Scilit:
- Direct stereochemical resolution of enantiomeric amides via thin-layer chromatography on a covalently bonded chiral stationary phaseJournal of Chromatography A, 1989
- The FDA perspective on the development of stereoisomersChirality, 1989
- Use of hydroxypropyl- and hydroxyethyl-derivatized β-cyclodextrins for the thin-layer chromatographic separation of enantiomers and diastereomersJournal of Chromatography A, 1988
- Planar chromatographic separation of enantiomers and diastereomers with cyclodextrin mobile phase additivesJournal of Chromatography A, 1988
- Thin-layer chromatographic separation of optical, geometrical, and structural isomersAnalytical Chemistry, 1986
- Thin‐Layer Chromatographic Enantiomeric Resolution via Ligand ExchangeAngewandte Chemie International Edition in English, 1984
- Enantiomeric separation of Dns-amino acids by reversed-phase thin layer chromatographyJournal of Chromatography A, 1984
- Resolution of optical isomers by thin layer chromatographyTetrahedron Letters, 1984
- Direct resolution of enantiomers via thin-layer chromatography using a chiral adsorbentJournal of Chromatography A, 1983
- A widely useful chiral stationary phase for the high-performance liquid chromatography separation of enantiomersJournal of the American Chemical Society, 1981