Evidence for an excited-state reaction contributing to NADH fluorescence
- 1 March 1995
- journal article
- Published by Springer Nature in Journal of Fluorescence
- Vol. 5 (1) , 99-106
- https://doi.org/10.1007/bf00718787
Abstract
The fluorescence of reduced β-nicotinamide adenine dinucleotide (NADH) was monitored as a function of the excitation and emission wavelengths. In aqueous and organic solvents the intensity decay was found to be more heterogeneous than reported earlier. When the ternary complex of NADH with the enzyme (horse liver alcohol dehydrogenase) and substrate analog (iso-butyramide) is formed, three exponents are required to fit the data. The decay-associated spectrum for the shortest lifetime undergoes a sign change from positive at the blue edge of emission to negative at the red edge. This phenomenon is interpreted as an outcome of reversible excited-state reaction leading to the appearance of at least one fluorescent product.Keywords
This publication has 16 references indexed in Scilit:
- Fluorescence study of a mutant cytochrome b5 with a single tryptophan in the membrane-binding domainBiochemistry, 1991
- Excited-state proton transfer of equilenin and dihydroequilenin: interaction with bilayer vesiclesBiochemistry, 1986
- Global analysis of fluorescence decay surfaces: excited-state reactionsChemical Physics Letters, 1985
- Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometryJournal of Biochemical and Biophysical Methods, 1981
- THE FLUORESCENCE DECAY OF REDUCED NICOTINAMIDES IN AQUEOUS SOLUTION AFTER EXCITATION WITH A UV‐MODE LOCKED Ar ION LASERPhotochemistry and Photobiology, 1981
- Analysis of two-state excited-state reactions. The fluorescence decay of 2-naphtholThe Journal of Physical Chemistry, 1979
- Pulse fluorimetry study of beef liver glutamate dehydrogenase-reduced nicotinamide adenine dinucleotide phosphate complexesBiochemistry, 1976
- Synthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. Studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, [reduced acetylpyridineadenine dinucleotide] and simplified synthetic modelsJournal of the American Chemical Society, 1970
- On the ternary complex of liver alcohol dehydrogenase with reduced coenzyme and isobutyramide. Effect of p-chloromercuriphenyl sulfonate and stability of the complexArchives of Biochemistry and Biophysics, 1962
- Dissociation constants of the liver alcohol dehydrogenase coenzyme complexesArchives of Biochemistry and Biophysics, 1959