Nucleotide sequence of cDNA clones of the murine myb proto-oncogene.

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Abstract
We have isolated cDNA clones of murine c‐myb mRNA which contain approximately 2.8 kb of the 3.9‐kb mRNA sequence. Nucleotide sequencing has shown that these clones extend both 5′ and 3′ to sequences homologous to the v‐myb oncogenes of avian myeloblastosis virus and avian leukemia virus E26. The sequence contains an open reading frame of 1944 nucleotides, and could encode a protein which is both highly homologous, and of similar size (71 kd), to the chicken c‐myb protein. Examination of the deduced amino acid sequence of the murine c‐myb protein revealed the presence of a 3‐fold tandem repeat of 52 residues near the N terminus of the protein, and has enabled prediction of some of the likely structural features of the protein. These include a high alpha‐helix content, a basic region toward the N terminus of the protein and an overall globular configuration. The arrangement of genomic c‐myb sequences, detected using the cDNA clones as probes, was compared with the reported structure of rearranged c‐myb in certain tumour cells. This comparison suggested that the rearranged c‐myb gene may encode a protein which, like the v‐myb protein, lacks the N‐terminal region of c‐myb.