SALMONELLA ISOLATION WITH RAPPAPORTS ENRICHMENT MEDIUM OF DIFFERENT COMPOSITIONS

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 173  (5) , 382-389
Abstract
There were 100 feces samples of normal pigs examined for the presence of salmonellae. The feces were pre-enriched for 18 h in peptone water, then 0.1 ml of the pre-enrichment medium was inoculated in 10 ml of the original Rappaport medium (R30) and 2 modifications (R25 and R10) of this medium. Two of the broths (R30 and R25) were incubated at 37.degree. C (R30/37.degree. C and R25/37.degree. C); the R10 medium was incubated at 43.degree. C (R10/43.degree. C). In addition, 100 ml of R10 medium (R10/100 ml/43.degree. C) and 100 ml of the standardized Muller Kauffmann''s tetrathionate broth (MK/100 ml/43.degree. C) were inoculated with 1 ml and 10 ml, respectively, of the pre-enrichment medium and incubated at 43.degree. C. With the method R10/100 ml/43.degree. C, 37 samples were found positive; with the method MK/100 ml/43.degree. C, only 22 positive samples were detected (P < 0.001). With the Rappaport media in 10 ml volumes, the modification R10 yielded 26 positive samples; the R30 and R25 broths yielded only 15 and 19 positive samples, respectively. All Rappaport procedures had a much stronger inhibition of the competing organisms, particularly those giving Salmonella-like colonies (lactose and sucrose negative), than did the MK/100 ml/43.degree. C method, a fact of considerable importance in everyday practice.

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