The effects of methylated thymidines upon cultures of V79 cells and the mechanism of incorporation of O4-methylthymidine into their DNA
- 31 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 3 (2) , 219-222
- https://doi.org/10.1093/carcin/3.2.219
Abstract
High concentrations of O2-methylthymidine, 3-methylthymidine and O4-methylthymidine (O4-MedT) have little effect on the colony forming ability of cultured Chinese hamster (V79) cells. However, the methylated derivatives enhance the toxic effects of thymidine (dT) at concentrations at which the modified nucleosides alone show no toxicity. O4-MedT was the only modified nucleoside which is incorporated into V79 cell DNA at a detectable level. In the presence of added dT there is an increase in the amount of O4-MedT incorporated. O4-MedT did not induce mutation, either forward or reverse, in V79 cells. O4-MedT is apparently incorporated into DNA as an analog of deoxycytidine and, once incorporated, behaves as cytidine during DNA synthesis, in contrast to O4-MedT produced in cellular DNA by methylating carcinogens, which is miscoding and potentially mutagenic.This publication has 2 references indexed in Scilit:
- DNA Methylation and Gene FunctionScience, 1980
- Reversion of 6-thioguanine resistant Chinese hamster cell lines: agent specificity and evidence for the repair of promutagenic lesionsCarcinogenesis: Integrative Cancer Research, 1980