VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A
- 1 December 1989
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 45 (11-12) , 1102-1105
- https://doi.org/10.1007/bf01950169
Abstract
At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63000 dalton binding protein in Cl. 19A cells.Keywords
This publication has 15 references indexed in Scilit:
- The Human Vasoactive Intestinal Peptide Receptor: Molecular Identification by Covalent Cross-Linking in Colonic Epithelium*Journal of Clinical Endocrinology & Metabolism, 1985
- Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system.Journal of Clinical Investigation, 1985
- Interaction of GRF with VIP receptors and stimulation of adenylate cyclase in rat and human intestinal epithelial membranesFEBS Letters, 1983
- Characterization of Vasoactive Intestinal Peptide Receptors in Human Colonic Epithelial Cells*Journal of Clinical Endocrinology & Metabolism, 1981
- Vasoactive intestinal peptide: A potent stimulator of adenosine 3′:5′-cyclic monophosphate accumulation in gut carcinoma cell lines in cultureProceedings of the National Academy of Sciences, 1978
- VASOACTIVE INTESTINAL PEPTIDE (VIP): VARIATION OF THE JEJUNO-ILEAL CONTENT IN THE DEVELOPING RAT AS MEASURED BY RADIORECEPTORASSAYActa Endocrinologica, 1977
- In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stainExperimental Cell Research, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970