Molecular analysis of the REV 2 gene ofSaccharomyces cerevisiae - a review
- 1 December 1990
- journal article
- research article
- Published by Springer Nature in Radiation and Environmental Biophysics
- Vol. 29 (4) , 293-301
- https://doi.org/10.1007/bf01210409
Abstract
The REV2 gene controls DNA repair, induced mutagenesis and, probably, some fidelity mechanism of replication. Of particular interest is the notion that it is inducible by DNA-damaging agents. We wanted to find molecular evidence for these results derived from numerous biological experiments. We cloned the REV2 gene from a yeast genomic DNA library based on the YCp 50 centromere vector, sequenced it and studied its regulation on the transcriptional level. The coding region of the REV2 gene consists of a 1425 pb reading frame with a coding capacity for a polypeptide of 52 kD; no significant homology to any gene filed in available data bases was found. Examination of a hydrophobicity plot of the putative Rev2 protein predicts the existence of transmembrane helices. Quantitative Northern analysis confirmed the working hypothesis that DNA-damaging agents increase the level of REV2 gene expression in stationary cells. Thus, the REV2 gene seems to code for a membrane protein which is inducible by DNA-damaging agents and which controls processes of repair and mutagenesis in yeast.Keywords
This publication has 37 references indexed in Scilit:
- Regulation of the RAD2 gene of Saccharomyces cervisiaeMolecular Microbiology, 1989
- Enhancement of DNA repair capacity of mammalian cells by carcinogen treatmentSomatic Cell and Molecular Genetics, 1988
- The RAD3 gene of Saccharomyces cerevisiae: Isolation and characterization of a temperature-sensitive mutant in the essential function and of extragenic suppressors of this mutantMolecular Genetics and Genomics, 1987
- The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzymeNature, 1987
- Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiaeCurrent Genetics, 1986
- A mismatch repair-based model can explain some features of u.v. mutagenesis in yeastMutagenesis, 1986
- Analysis of non-linearities in mutation frequency curvesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- Inducibility of error-prone DNA repair in yeast?Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1984
- Mutagenesis in Saccharomyces CerevisiaeAdvances in Genetics, 1982
- Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligaseNature, 1978