Excretion patterns of urinary enzymes having amidolytic and esterolytic activities in the urine of male and female rats.

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Abstract
Excretion patterns of urinary enzymes having amidolytic and esterolytic activities were examined in the urine of male and female rats. Kallikrein and non-kallikrein [esterase A1 (EA1) and esterase A2 (EA2)] fractions were separated by diethylaminoethyl-cellulose chromatography and amidolytic and esterolytic activities were assayed by using prolyl-phenylalanyl-arginine-4-methylcoumaryl-7-amide and N-α-tosyl-L-arginine methyl ester. In male rat urine, about 25% of total esterolytic activity was due to kallikrein, and the remainder was attributable to EA1 and EA2. In female rat urine, about 45% of total esterolytic activity was due to kallikrein and the remainder was mostly attributable to EA2. EA1 in female rats accounted for less than one-tenth of total esterolytic activity. On the other hand, about 87 and 13% of total amidolytic activity were due to kallikrein and EA2, respectively, in both male and female rats. However, EA1 had no amidolytic activity. These findings indicate that most of the urinary amidolytic activity in both sexes is associated with kallikrein, in contrast to urinary esterolytic activity, and that EA1 is not relevant to the results of urinary amidolytic assay.