Activity of Fructose-1,6-Diphosphatase in Human Leukocytes
- 12 June 1975
- journal article
- Published by Massachusetts Medical Society in New England Journal of Medicine
- Vol. 292 (24) , 1298-1299
- https://doi.org/10.1056/nejm197506122922420
Abstract
To the Editor: Melancon and Nadler1 2 3 reported the presence of fructose-1, 6-diphosphatase (FDPase) activity in human leukocytes, a finding that could not be confirmed by Cahill and Kirtley.4 Because leukocytes are more convenient to obtain than a liver biopsy, we should like to report an assay procedure by which we were able to detect a FDPase deficiency in leukocytes of a patient who already had the diagnosis of FDPase deficiency in the liver.Homogenates of leukocytes and liver were prepared in 10 mM Tris-hydrochloride (pH 7.4), 1 mM EDTA and 0.02 per cent Triton X-100. The leukocytes were disintegrated by . . .Keywords
This publication has 4 references indexed in Scilit:
- Insignificance of Gluconeogenesis in Human Blood PlateletsEuropean Journal of Clinical Investigation, 1975
- Metabolic and biochemical studies in fructose 1, 6-diphosphatase deficiencyThe Journal of Pediatrics, 1973
- Hepatic fructose-1,6-diphosphatase deficiencyJournal of Clinical Investigation, 1972
- Detection of Fructose-1,6-Diphosphatase Deficiency with Use of White Blood CellsNew England Journal of Medicine, 1972