Inhibition of mutant troponin C activity by an intra-domain disulphide bond
- 1 May 1990
- journal article
- Published by Springer Nature in Nature
- Vol. 345 (6271) , 132-135
- https://doi.org/10.1038/345132a0
Abstract
Triggering of contraction in striated muscles involves a conformational transition in the N-terminal domain of troponin C, the calcium-binding component of thin filaments. We have designed a mutant troponin C in which the key conformational transition and the calcium-regulatory activity are reversibly blocked by the formation of a disulphide bridge. Our results may be applicable to other proteins of the same family of calcium-binding proteins.Keywords
This publication has 34 references indexed in Scilit:
- Characterization of zero-length cross-links between rabbit skeletal muscle troponin C and troponin I: evidence for direct interaction between the inhibitory region of troponin I and the amino-terminal, regulatory domain of troponin CBiochemistry, 1990
- Refined crystal structure of troponin C from turkey skeletal muscle at 2·0 Å resolutionJournal of Molecular Biology, 1988
- Comparison of the crystal and solution structures of calmodulin and troponin CBiochemistry, 1988
- pH-dependent structural transition in rabbit skeletal troponin C.Published by Elsevier ,1987
- Structural Aspects of Troponin-Tropomyosin Regulation of Skeletal Muscle ContractionAnnual Review of Biophysics, 1987
- pH-DEPENDENT CHANGES IN THE SPATIAL RELATIONSHIP BETWEEN THE DOMAINS OF TROPONIN C11Supported by grants from NIH (AR32727, AR21673, HL05949 and2RR05711), MDA, and AHA (Massachusetts Affiliate).Published by Elsevier ,1987
- Modulation of the interaction between the two halves of troponin C by the other troponin subunitsEuropean Journal of Biochemistry, 1986
- Stabilization of the long central helix of troponin C by intrahelical salt bridges between charged amino acid side chains.Proceedings of the National Academy of Sciences, 1985
- Thin Filament Proteins and Thin Filament-Linked Regulation of Vertebrate Muscle ContractioCritical Reviews in Biochemistry, 1984
- The time-course of Ca2+ exchange with calmodulin, troponin, parvalbumin, and myosin in response to transient increases in Ca2+Biophysical Journal, 1981