Chemical Structure of Glycolipid of Guinea Pig Red Blood Cell Membrane
Open Access
- 1 April 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 75 (4) , 837-842
- https://doi.org/10.1093/oxfordjournals.jbchem.a130455
Abstract
A hexosamine-containing glycolipid, comprising about 6.8% of the total lipids, was extracted and purified from the guinea pig red cell membrane. The carbohydrate moiety consisted of glucose, galactose, and N-acetylgalactosamine in equimolar proportions. Analysis of the sugar linkages by methylation of this glycolipid indicated that the C-4 positions of glucose and galactose were substituted by another sugar and N-acetylgalactosamine resided on the non-reducing terminal. The configuration of the glycosidic linkages was established by optical rotation measurements, PMR spectra and specific enzymatic hydrolysis. The structure of this glycolipid was shown to be N-acetylgalactosaminyl β(1→4)-galactosyl-β(1→4)-glucosyl-(1→1)-ceramide. The major fatty acid was C24:0, and the long chain base was mostly 4-sphingenine.Keywords
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