Disulfide Bridges of Boviae Spleen Cathepsin B
- 1 January 1990
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 371 (1) , 485-492
- https://doi.org/10.1515/bchm3.1990.371.1.485
Abstract
Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.This publication has 12 references indexed in Scilit:
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