PURIFICATION OF THE CYTOPLASMIC OESTROGEN RECEPTOR FROM MAMMARY TUMOURS INDUCED IN RATS WITH DIMETHYLBENZANTHRACENE
- 1 April 1979
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 81 (1) , 119-130
- https://doi.org/10.1677/joe.0.0810119
Abstract
The oestrogen receptor from mammary tumours induced in rats by dimethylbenz(a)-anthracene has been extensively purified by affinity chromatography and isoelectric focusing. The unpurified cytoplasmic 8 S oestrogen receptor had a molecular weight of 240 000, a Stokes radius of 5·4 nm and a frictional ratio of 1·32; the Km (dissociation constant) at 4 °C for [3H]oestradiol-17β was 0·184 nmol/l. At the end of affinity chromatography the molecular weight was still 240 000 but under the conditions of isoelectric focusing it was reduced to 110 000, with a Stokes radius of 4·0 nm, a frictional ratio of 1·26 and an isoelectric point of 6·4.This publication has 2 references indexed in Scilit:
- Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductasesPublished by Elsevier ,2003
- Affinity Chromatography and the Purification of Estrogen ReceptorsJournal of Biological Chemistry, 1973