Mutation of the p53 Gene in Gallbladder Cancer.
Open Access
- 1 January 1994
- journal article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 172 (3) , 283-289
- https://doi.org/10.1620/tjem.172.283
Abstract
TAKAGI, S., NAITO, E., YAMANOUCHI, H., OHTSUKA, H., KOMINAMI, R. and YAMAMOTO, M. Mutation of the p53 Gene in Gallbladder Cancer. Tohoku J. Exp. Med., 1994, 172 (3), 283-289-Genetic alteration of the p53 gene was examined in tissue specimens of primary gallbladder cancer (GBC) using PCR-single strand conformation polymorphism (SSCP) and direct sequencing analyses. The p53 gene mutation was detected in five of the 16 GBC cases examined (31%). All five cases showed single point mutations; four were mutations resulting in amino acid substitution and the other was one base-pair deletion. Of the five mutations detected, four occurred at evolutionarily conserved regions and the remaining one at a well conserved region among species. Our result is similar to those of previous reports for various human cancers in mutation scattering over four of the five conserved domains.Keywords
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