FACTORS AFFECTING HEPATIC GLYCOLYSIS AND SOME CHANGES THAT OCCUR DURING DEVELOPMENT

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Abstract
Glycolysis by the supernatant fraction of homogenates of liver from guinea pigs and rats at various stages of development (fetal, newborn and adult) has been examined in a suitably fortified medium by measurement of inorganic phosphate uptake and production of lactate and glycerol 1-phosphate. Starting with glucose as substrate, 2 rate-determining steps in glycolysis occur at the stages of glucose phosphorylation and the phosphofructokinase reaction in liver from animals of all ages. Effects of the post-natal development of glucokinase are recorded. The appearance of microsomal glucose 6-phosphatase activity around birth has an effect on glycolysis owing to competition for glucose 6-phosphate. A stimulating effect of the nuclear fraction, especially from fetal liver, on glycolysis by the supernatant fraction is interpreted as being due to stimulation by adenosine-triphosphatase activity at the 3-phosphoglycerate-kinase stage.

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