Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase
- 1 October 1978
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 275 (5681) , 617-624
- https://doi.org/10.1038/275617a0
Abstract
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host [Escherichia coli] cells resistant to the antimetabolic drug trimethoprim.This publication has 46 references indexed in Scilit:
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