Some kinetic and molecular properties of yeast phosphofructokinase
- 1 August 1968
- journal article
- Published by Wiley in FEBS Letters
- Vol. 1 (3) , 137-141
- https://doi.org/10.1016/0014-5793(68)80041-9
Abstract
Yeast PFK had a sedimentation coefficient of 16.7 S both in the absence and in the presence of ATP, and did not dissociate even at very low protein concentrations. Sodium dodecyl-sulphate caused dissociation of the protein to sub-units of 3.2 S. The effects of pH on substrate affinities are described. In the presence of UTP, acting as non-inhibiting phosphate donor, the behaviour of the enzyme towards F-6-P was co-operative, with a Hill coefficient of 2.2.Keywords
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