GENETIC-POLYMORPHISM OF PLATELET GLYCOPROTEIN-IB

  • 1 January 1984
    • journal article
    • research article
    • Vol. 64  (3) , 622-629
Abstract
Platelet glycoprotein (GP)lb from 131 healthy Japanese was analyzed using SDS [sodium dodecyl sulfate]-polyacrylamide gel electrophoresis and specific staining with peroxidase-coupled wheat germ agglutinin after it was transferred to nitrocellulose membranes. Four slightly different species of GPlb were observed and designated as A, B, C and D for glycoproteins with MW 168,000, 162,000, 159,000 and 153,000 daltons, respectively. The respective gene frequencies were calculated to be 0.073, 0.011, 0.561 and 0.355 for A-, B-, C- and D-type GPlb. Portions from each type of GPlb molecule (.alpha.-chain and glycocalicin) showed heterogeneity with the same MW differences, indicating that the variance would be derived from the polypeptide portion that is exposed to the outer medium. The different types of GPlb were the same with respect to their accessibility to lactoperoxidase, reactivity to lectins and affinity to TLCK [tosyl-L-lysine-chloromethyl ketone]-thrombin. Although patients were reported with a bleeding tendency whose platelets have double GPlb bands platelets with different GPlb phenotypes showed no significant differences in aggregating activity and platelet retention. Analysis of GPlb phenotype should be important for structural and physiologic studies on GPlb and glycocalicin.

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