Restriction map of the single-stranded DNA genome of Kilham rat virus strains 171, a nondefective parvovirus

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Abstract
A physical map of Kilham rat virus strain 171 DNA was constructed by analyzing the sizes and locations of restriction endonuclease-generated fragments of the replicative-form viral DNA synthesized in vitro. BglI, KpnI, BamHI, SmAI, XhoI and XorII did not appear to have any cleavage sites; 11 other enzymes cleaved the genome at 1-8 sites, and AluI generated > 12 distinct fragments. The 30 restriction sites that were mapped were distributed randomly in the viral genome. A comparison of the restriction fragments of in vivo- and in vitro-replicated replicative-form DNA showed that these DNA were identical except in the size or configuration of the terminal fragments.