A Study of Tobacco Carcinogenesis. XX. Role of Catechol as a Major Cocarcinogen in the Weakly Acidic Fraction of Smoke Condensate2, 3, 4

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Abstract
The weakly acidic fraction ot Cigarette smoke condensate was fractionated by preparative high-pressure liquid chromatography into major subtractions I-IV. Major subfractions II and III were fractionated further into subfractions A-J. Subfractions A-J were tested tor cocarcinogenicity on the skin of noninbred Ha:ICR Swiss albino mice by application with 0.003% benzo[a)pyrene. Subtractions A-C and F-J showed signiticant cocarcinogenic activity; subtractions A, F, and H were the most active. Catechol was a major component of subtraction A and was also detected in subfractions B-D and F. Major components of the other subtractions included hydroquinone (8), coniferyl alcohol (C and H), hydroxyphenyl alcohols (D), alkyl-2-hydroxy-2-cyclopenten-1-ones (C, D, and F), hydroxyacetophenones (F), phenolic cyano compounds (F), and fatty acids (F). The results demonstrate the importance of catechol as a cocarcinogen in the weakly acidic traction of cigarette smoke condensate and indicate the presence of other cocarcinogens.