CLONAL REARRANGEMENT OF THE BETA-T-CELL RECEPTOR GENE IN MULTIPLE-MYELOMA
- 1 February 1989
- journal article
- research article
- Vol. 3 (2) , 133-136
Abstract
Because clonal rearrangements of the .beta.-T cell receptor (.beta.-TCR) gene occur in some patients with B cell chronic lymphocytic leukemia, we studied the arrangement of this gene in fourteen patients with multiple myeloma, a malignancy of the most terminally differentiated B cells. The gene was in germline configuration in peripheral blood lymphocytes (PBLs) and bone marrow samples of thirteen patients. By contrast, it was clonally rearranged in the marrow but not in the PBLs of one patient with stage IIA IgA-.lambda. myeloma. This patient''s bone marrow consisted of 95% morphologically identifiable plasma cells which were CALLA-, OKT10+ (93%), and PCA-1+ (78%). Only 5% of marrow cells were small lymphocytes which contained T cell markers (CD3+ or CD2+). To eliminate the possibility that the small percentage of contaminating T cells contained the gene rearrangement, they were depleted by avidin-blotin immunoadsorption using the Leu4 determinant. Positively selected marrow T cells did not contain .beta.-TCR gene rearrangements. By contrast, the T cell depleted marrow contained the rearranged gene. This is the first demonstration that rearranged .beta.-TCR genes can occur in multiple myeloma.This publication has 12 references indexed in Scilit:
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