Chemical Mutagenesis of Fusarium oxysporum f. sp. lycopersici: Non-selected Changes in Pathogenicity of Auxotrophic Mutants

Abstract
SUMMARY: Single and multiple auxotrophic mutants of the Fusarium oxysporum f. sp. lycopersici strains which cause Fusarium crown-rot and Fusarium wilt of tomato were obtained chemical mutagenesis with nitrous acid, nitrosoguanidine and ethyl-methanesulphonate. The mutagenesis and selection procedures, adapted for use with a plant pathogenic fungus, are described. Changes in pathogenicity were observed when the auxotrophs were compared with the wild type but no correlation was observed between changes in pathogenicity and the particular nutritional requirement.

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