Escherichia coli gene that controls sensitivity to alkylating agents
- 1 July 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 135 (1) , 144-152
- https://doi.org/10.1128/jb.135.1.144-152.1978
Abstract
A new type of E. coli mutant which shows increased sensitivity to methyl methane sulfonate but not to UV light or to .gamma. rays was isolated after mutagenesis with N-methyl-N''-nitro-N-nitrosoguanidine. The mutant is unable to reactivate phage .lambda.vir or double-stranded .vphi.X174 DNA (replicative form) that was treated with methyl methane sulfonate. The mutant is also sensitive to other alkylating agents, such as ethyl methane sulfonate, mitomycin C and N-methyl-N''-nitro-N-nitrosoguanidine. It grows normally and exhibits almost normal recombination proficiency. The mutant possesses normal levels of DNA polymerase I, exonuclease I, exonuclease V, endonuclease specific for methyl methane sulfonate-treated DNA and 3-methyladenine-DNA glycosidase activities. The genetic locus responsible was named alk and is located near his on the chromosome.This publication has 21 references indexed in Scilit:
- Studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage T4: The structural gene for T4 endonuclease VJournal of Molecular Biology, 1976
- New class of enzymes acting on damaged DNANature, 1976
- Action of exonuclease V (the recBC enzyme) on ultraviolet-irradiated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Some chemical aspects of dose-response relationships in alkylation mutagenesisMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
- Mutants of Escherichia coli with altered deoxyribonucleases: II. Isolation and characterization of mutants for exonuclease IJournal of Molecular Biology, 1974
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- Genetic Analysis of an E. coli Strain with a Mutation affecting DNA PolymeraseNature, 1969
- ENDONUCLEASE II OF E. coli , I. ISOLATION AND PURIFICATIONProceedings of the National Academy of Sciences, 1969
- Chromatographic studies with immobilized polynucleotide in acrylamide gel matrixBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- Transduction of linked genetic characters of the host by bacteriophage P1Virology, 1955