IMMUNOHISTOCHEMICAL LOCALIZATION OF CATECHOLAMINE SYNTHESIZING ENZYMES IN BULLFROG ADRENALS

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Abstract
Frog adrenal chromaffin cells have very long processes. These chromaffin cells are scattered in cortical lipid cells and sometimes in summer cells. By immunofluorescent tyrosine hydroxylase(TH)-positive, dopamine-.beta.-hydroxylase(DBH)-positive, phenylethanolamine-N-methyltransferase(PNMT)-positive adrenaline cells, and TH-positive, DBH-positive, PNMT-negative noradrenaline cells were located in frog adrenal glands. The activities of TH, DBH and PNMT in the adrenals of the bullfrogs were 215, 22,905 and 302 pmol/min per mg protein, respectively. Acidophilic summer cells have neither TH, DBH nor PNMT. These results were reconfirmed by fluorescence microspectrophotometry.