STUDIES ON THE TECHNIQUE OF VITREOUS FLUOROPHOTOMETRY

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 22  (5) , 668-674
Abstract
The sources of artifacts of vitreous fluorophotometry (VF), a technique used to assess the permeability of the blood-retinal barrier, was examined. By performing in vitro measurements in a model eye and in vivo readings in humans, the influence of various concentrations of fluorescein in the anterior chamber and in the retina on the measurements performed in the vitreous was investigated. Upper limits for the artifactual effects are set under clinical conditions, and a way to account for them is proposed. Special attention is given to depth resolution, which was investigated as a function of the region being scanned, the beam width, and the probe size. Preliminary results indicate that a precedure to account for light attenuation by the lens is feasible. Apparently, reliable quantitative results can be obtained by VF, provided that the artifacts are acknowledged, minimized, and corrected.